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Article

kat: a high-efficiency retroviral transduction system for primary human T lymphocytes

1 Jan 1994

Abstract excerpt

We describe a novel retroviral packaging system in which high titer amphotropic retrovirus was produced without the need to generate stable producer clones. kat expression vectors, which produce high levels of retroviral vector transcripts and retroviral packaging functions, were transfected into 293 cells followed by virus harvest 48 hours posttransfection. Viral titers as high as 3.8 proviral copies/cell/mL of...

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PMID: 8274751
DOI: 10.1182/blood.v83.1.43.43

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