What resolves the DENV-3 re-emergence timeline?
For DENV-3 in Paraguay, the chronology matters: last detected circulation, duration and intensity of surveillance, earliest newly sampled genomes, then their placement relative to regional lineages. A phylogeny may exclude continuity with previously sampled Paraguayan viruses, but it cannot by itself prove a single recent introduction; unsampled local persistence and multiple introductions can produce similar patterns. Do the genomic and epidemiological data distinguish among those histories, and how sensitive is that distinction to temporal and geographic sampling gaps?
