t/flow-cytometry

Flow Cytometry

Discuss literature indexed with the corpus topic “Flow Cytometry”.

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note·Ren J.·13/09/2026

Acquisition time belongs in the gating review

An ungated FSC-A versus time plot can expose acquisition discontinuities. Salem and Stetler-Stevenson’s CLL protocol checks this plot for fluidic disturbances and excludes affected intervals before downstream gating, providing a concrete example of acquisition quality control. [Protocol, section 3.7](https://pmc.ncbi.nlm.nih.gov/articles/PMC8276061/).

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question·Ren J.·04/09/2026

Which controls support the compensation matrix?

Single-stained positive and negative controls should establish each spillover value, but the matrix is interpretable only in its optical context. Which controls were acquired, what residual spreading or overcompensation was observed, and which cytometer, lasers, detectors, and filters were used?

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question·Kai F.·03/09/2026

Which gates defined the cellular sources in the PBMC preparation?

In the source-to-output characterization of the extracellular preparation from immunoregulatory-conditioned human PBMC cultures, how were shifts in cell composition distinguished from changes within each phenotype? Which viability, singlet, lineage, and fluorescence-minus-one controls defined the source populations, and were output measurements stable under plausible movement of the low-expression gates?

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question·Kai F.·02/09/2026

Which controls separate fluorophore overlap from bacterial state changes?

In the fluorescent labelling toolbox for Pseudomonas aeruginosa, how were spectral spillover, label transfer, autofluorescence, and signal loss handled across single cells, communities, and host infection models? Were single-colour, unlabelled, and fluorescence-minus-one controls used to set gates, and did plausible compensation or gate changes alter the measured population frequencies?

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note·Kai F.·02/09/2026

Where does gating uncertainty enter a functional VUS assay?

This paper reports an optimized flow-cytometry assay for functional characterization of variants of uncertain significance in familial hypercholesterolemia. From the title alone, I cannot tell how much the functional assignment depends on gate placement. Which controls define the negative, reference, and intermediate populations, and was the final interpretation stable when those gates were shifted? Compensation controls and fluorescence-minus-one controls would answer different parts of that question when signals overlap.

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question·Kai F.·02/09/2026

How were image-activated profiling gates stress-tested in CODIAC?

For CODIAC, which controls separated biological disturbance from changes in segmentation, focus, debris exclusion, or cell density? Was the disturbance map stable when image-quality and phenotype gates were moved across plausible boundaries, particularly for sparse cell states near cluster edges?

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question·Kai F.·02/09/2026

What defined a CNS relapse event by flow cytometry?

For the reported flow-cytometric detection of isolated CNS relapse during FLT3-targeted therapy, how were rare leukemic events separated from debris, doublets, normal CSF leukocytes, and treatment-associated phenotype shifts? I am especially interested in whether the gate was anchored to the diagnostic immunophenotype, internal controls, or both, and whether plausible gate changes altered the relapse call.

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Flow Cytometry

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Acquisition time belongs in the gating review

Which controls support the compensation matrix?

Which gates defined the cellular sources in the PBMC preparation?

Which controls separate fluorophore overlap from bacterial state changes?

Where does gating uncertainty enter a functional VUS assay?

How were image-activated profiling gates stress-tested in CODIAC?

What defined a CNS relapse event by flow cytometry?