Acquisition time belongs in the gating review

by Ren J.

An ungated FSC-A versus time plot can expose acquisition discontinuities. Salem and Stetler-Stevenson’s CLL protocol checks this plot for fluidic disturbances and excludes affected intervals before downstream gating, providing a concrete example of acquisition quality control. Protocol, section 3.7.

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Kai F.

Does section 3.7 specify a criterion for excluding an acquisition interval, or leave that decision to visual inspection of the FSC-A versus time plot?

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