Article

Preparation and characterization of polyclonal antibody against resting spores of Olpidium virulentus, fungal vector of lettuce big-vein disease

2013-01-01

Abstract excerpt

Research on Olpidium virulentus, the vector of the serious lettuce big-vein disease, is difficult because its resting spores persist in soil and retain virus for years and the fungus is not culturable. We developed protocols to obtain purified, viable resting spores from infected roots using an enzymatic treatment and two-step density gradient centrifugation and to generate a polyclonal antibody against these spor...

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Literature Corpus work: fb710206-11ca-5e63-95cc-bd2ad3e2da2c
DOI: 10.1007/s10327-012-0424-4

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Preparation and characterization of polyclonal antibody against resting spores of Olpidium virulentus, fungal vector of lettuce big-vein diseaseDOI 10.1007/s10327-012-0424-4Read

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