Article

Mining the Proteome of Human Ovarian Cancer Extracellular Vesicles Using Thermolysin Proteolysis

2024-10-11

Abstract excerpt

This study investigates the utility of Thermolysin as a proteolytic enzyme to enhance the depth and coverage of proteomic analysis in ovarian cancer (OC) extracellular vesicles (EVs). EVs were isolated from OC cell lines and ascites fluid samples from women diagnosed with high-grade serous carcinoma. Proteins were digested using Thermolysin and Trypsin/LysC, followed by label-free data-dependent acquisition (DDA)...

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Literature Corpus work: fa804bc9-4196-5a58-aac4-d73075d76593
DOI: 10.1101/2024.10.10.617562

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