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Single- and duplex TaqMan-quantitative PCR for determining the copy numbers of integrated selection markers during site-specific mutagenesis in <i>Toxoplasma gondii</i> by CRISPR-Cas9

2022-06-23

Abstract excerpt

Herein, we developed a single and a duplex TaqMan quantitative qPCR for absolute quantification of copy numbers of integrated dihydrofolate reductase-thymidylate synthase ( mdhfr-ts ) drug selectable marker for pyrimethamine resistance in Toxoplasma gondii knockouts (KOs). The single TaqMan qPCR amplifies a 174 bp DNA fragment of the inserted mdhfr-ts and of the wild-type (WT) dhfr-ts ( wt-dhfr-ts ) which is...

Identifiers and source

Literature Corpus work
f314e645-e64c-5a3d-9303-d08ac393dd91
DOI
10.1101/2022.06.23.497358
Open publication

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Single- and duplex TaqMan-quantitative PCR for determining the copy numbers of integrated selection markers during site-specific mutagenesis in <i>Toxoplasma gondii</i> by CRISPR-Cas9DOI 10.1101/2022.06.23.497358
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