Article

Machine Learning-Guided Engineering of High-Affinity Cross-Reactive Antibodies with Minimal Mutations

2026-07-16

Abstract excerpt

Antibodies raised against human targets often fail to recognize their animal orthologs, limiting preclinical evaluation in relevant models. We developed a Deep Mutational Scanning (DMS)-coupled deep learning strategy to engineer potent cross-reactive antibodies with minimal sequence divergence. Starting from C4, a fully human anti-PD-L1 antibody with weak recognition of murine PD-L1, DMS identified substitutions t...

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Literature Corpus work: f161c085-7ecb-53b1-84c3-d8e237e8782c
DOI: 10.64898/2026.07.15.738714

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