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Establishment of a Duplex Fluorescent Quantitative PCR Assay for Staphylococcus aureus Detection Based on Pangenome Analysis

2025-07-17

Abstract excerpt

(1) Background: Staphylococcus aureus is a common human respiratory pathogen. Its widespread antimicrobial resistance often leads to severe infections. However, the high genomic diversity among strains frequently causes false negatives in conventional PCR assays. (2) Methods: To address this limitation, we developed a highly sensitive and specific duplex fluorescent quantitative PCR (qPCR) method for S. aureus det...

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Literature Corpus work
ea83d8f9-1600-55f4-8a94-a26ed0ac5e9a
DOI
10.20944/preprints202507.1490.v1
Open publication

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Establishment of a Duplex Fluorescent Quantitative PCR Assay for Staphylococcus aureus Detection Based on Pangenome AnalysisDOI 10.20944/preprints202507.1490.v1
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