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Title Detection of Pathogens by a Novel User-Developed Broad-Range BR 16S PCR rRNA Polymerase Chain Reaction/Gene Sequencing Assay: Multiyear Experience in a Large Canadian Healthcare Zone

2025-07-03

Abstract excerpt

Between 2015 and 2022, we evaluated a novel BR 16S PCR rDNA PCR/Sanger sequencing assay to improve diagnosis of invasive infections in culture-negative specimens. Using dual-priming oligonucleotide (DPO), this assay analyzed ribo-somal DNA from sterile fluids or tissues. A total of 772 specimens from 661 patients were studied: 425 specimens (61%) had negative culture and BR 16S PCR tests; 245 specimens (35%) had n...

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Literature Corpus work
ee1bcadf-3806-5709-8c3d-fbdec00989da
DOI
10.20944/preprints202507.0289.v1
Open publication

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