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Article

Rapid Sanger Sequencing of the 16S rRNA Gene for Identification of Some Common Pathogens

14 Feb 2014

Abstract excerpt

Conventional Sanger sequencing remains time-consuming and laborious. In this study, we developed a rapid improved sequencing protocol of 16S rRNA for pathogens identification by using a new combination of SYBR Green I real-time PCR and Sanger sequencing with FTA® cards. To compare the sequencing quality of this method with conventional Sanger sequencing, 12 strains, including three kinds of strains (1 reference...

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PMID: 24551186
DOI: 10.1371/journal.pone.0088886

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