Article

Extraction-free protocol combining Proteinase K and heat inactivation for detection of SARS-CoV-2 by RT-qPCR

2020-12-17

Abstract excerpt

Real-time reverse transcription PCR (RT-qPCR) is the gold-standard technique for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection in nasopharyngeal swabs specimens. The analysis by RT-qPCR usually requires a previous extraction step to obtain the purified viral RNA. Unfortunately, RNA extraction constitutes a bottleneck for early detection in many countries since it is expensive, time-consumi...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: e81b7041-eae9-5a17-8199-bffe2078214b
DOI: 10.1101/2020.12.16.20248350

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Extraction-free protocol combining Proteinase K and heat inactivation for detection of SARS-CoV-2 by RT-qPCRDOI 10.1101/2020.12.16.20248350Read

A streamlined approach to rapidly detect SARS-CoV-2 infection, avoiding RNA purificationSemantic proximity Read

A simple RNA preparation method for SARS-CoV-2 detection by RT-qPCRSemantic proximity Read

Rapid, Reliable and Robust approach for extraction-free RT-PCR based detection of SARS-CoV-2 in clinical setting to expedite large scale screeningSemantic proximity Read

Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extractionSemantic proximity Read

Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extractionSemantic proximity Read

Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivationSemantic proximity Read

Ultrafast RNA extraction-free SARS-CoV-2 detection by direct RT-PCR using a rapid thermal cycling approachSemantic proximity Read

Classic RNA extraction is efficient for subsequent detection of SARS-CoV-2 by RT-PCR in nasopharyngeal swab samplesSemantic proximity Read

SARS-CoV-2 detection using isothermal amplification and a rapid, inexpensive protocol for sample inactivation and purificationSemantic proximity Read

Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCRSemantic proximity Read

Rapid direct nucleic acid amplification test without RNA extraction for SARS-CoV-2 using a portable PCR thermocyclerSemantic proximity Read

Open-source RNA extraction and RT-qPCR methods for SARS-CoV-2 detectionSemantic proximity Read