Article

Multiplex Sequencing of SARS-Cov-2 genome directly from clinical samples using the Ion Personal Genome Machine (PGM)

2021-02-08

Abstract excerpt

<title>Abstract</title> <p>Various methods have been developed for rapid and high throughput full genome sequencing of SARS-CoV-2. Here, we described a protocol for targeted multiplex full genome sequencing of SARS-CoV-2 genomic RNA directly extracted from human nasopharyngeal swabs using the Ion Personal Genome Machine (PGM). This protocol involves concomitant amplification of 237 gene fragments encompassing the...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: e0bb4b1a-aef2-5a80-a1ca-73738ac4e8a6
DOI: 10.21203/rs.3.rs-141611/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Multiplex Sequencing of SARS-Cov-2 genome directly from clinical samples using the Ion Personal Genome Machine (PGM)DOI 10.21203/rs.3.rs-141611/v1Read

Evaluation of the Ion AmpliSeq SARS-CoV-2 Research Panel by Massive Parallel SequencingSemantic proximity Read

Two-Pool Multiplex Long-Amplicon Amplification and Full-Genome Sequencing of SARS-CoV-2Semantic proximity Read

Direct RNA nanopore sequencing of SARS-CoV-2 extracted from critical material from swabsSemantic proximity Read

Assessment of two‐pool multiplex long‐amplicon nanopore sequencing of SARS‐CoV‐2Semantic proximity Read

Performance of COVIDSeq and Swift normalase amplicon SARS-CoV-2 panels for SARS-CoV-2 Genomes Sequencing: Practical Guide and Combining FASTQ StrategySemantic proximity Read

Development and Validation of a High-Throughput Next-Generation Sequencing Workflow for SARS-CoV-2 Whole Genome Sequencing: Results from Over 65,000 Clinical CasesSemantic proximity Read

Evaluation of NGS-based approaches for SARS-CoV-2 whole genome characterisationSemantic proximity Read

SARS-CoV-2 genomes recovered by long amplicon tiling multiplex approach using nanopore sequencing and applicable to other sequencing platformsSemantic proximity Read

Next generation sequencing of SARS-CoV-2 genomes: challenges, applications and opportunitiesSemantic proximity Read

Comparison of SARS-CoV-2 sequencing using the ONT GridION and the Illumina MiSeqSemantic proximity Read

Analytical validity of nanopore sequencing for rapid SARS-CoV-2 genome analysisSemantic proximity Read

Development and validation of a high throughput SARS-CoV-2 whole genome sequencing workflow in a clinical laboratory.Semantic proximity Read