Article

Two-Pool Multiplex Long-Amplicon Amplification and Full-Genome Sequencing of SARS-CoV-2

2020-11-10

Abstract excerpt

<title>Abstract</title> <p>BackgroundSince the first public genome of SARS-CoV-2, over 170,000 genome sequences of the virus have been shared by researchers world-wide (till November 1st 2020). Multiplex PCR targeting SARS-CoV-2 followed by massively parallel sequencing (MPS) and/or nanopore sequencing is a widely used strategy to recover the genome from primary samples. However, the bias of amplification among d...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 53e510d9-ce67-5afc-bb14-c8995ffcd021
DOI: 10.21203/rs.3.rs-103455/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Two-Pool Multiplex Long-Amplicon Amplification and Full-Genome Sequencing of SARS-CoV-2DOI 10.21203/rs.3.rs-103455/v1Read

Assessment of two‐pool multiplex long‐amplicon nanopore sequencing of SARS‐CoV‐2Semantic proximity Read

Molecular Genetic Analysis of SARS-CoV-2 Lineages in ArmeniaSemantic proximity Read

AmpliCoV: Rapid Whole-Genome Sequencing Using Multiplex PCR Amplification and Real-Time Oxford Nanopore MinION Sequencing Enables Rapid Variant Identification of SARS-CoV-2Semantic proximity Read

Evaluation of two SARS-CoV-2 whole genome amplification and sequencing workflows for genomic surveillance and outbreak analysis in the clinical microbiology laboratory.Semantic proximity Read

Development and Validation of a High-Throughput Next-Generation Sequencing Workflow for SARS-CoV-2 Whole Genome Sequencing: Results from Over 65,000 Clinical CasesSemantic proximity Read

Rapid and Inexpensive Whole-Genome Sequencing of SARS-CoV-2 using 1200 bp Tiled Amplicons and Oxford Nanopore Rapid BarcodingSemantic proximity Read

Simplified Point-of-Care Full SARS-CoV-2 Genome Sequencing Using Nanopore TechnologySemantic proximity Read

Analytical validity of nanopore sequencing for rapid SARS-CoV-2 genome analysisSemantic proximity Read

Performance of COVIDSeq and Swift normalase amplicon SARS-CoV-2 panels for SARS-CoV-2 Genomes Sequencing: Practical Guide and Combining FASTQ StrategySemantic proximity Read

Rapid Genomic Characterization of SARS-CoV-2 Viruses From Clinical Specimens Using Nanopore SequencingSemantic proximity Read

Performance of amplicon and capture based next-generation sequencing approaches for the epidemiological surveillance of Omicron SARS-CoV-2 and other variants of concernSemantic proximity Read

Evaluation of NGS-based approaches for SARS-CoV-2 whole genome characterisationSemantic proximity Read