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Tools, strains, and strategies to effectively conduct anaerobic and aerobic transcriptional reporter screens and assays in <i>Staphylococcus aureus</i>

2021-06-13

Abstract excerpt

Transcriptional reporters are reliable and time-tested tools to study gene regulation. In Staphylococcus aureus , β-galactosidase ( lacZ )-based genetic screens are not widely used because of the necessity of selectable markers for strain construction and the production of staphyloxanthin pigment which obfuscates results. We describe a series of vectors that allow for markerless insertion of codon-optimized lac...

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Literature Corpus work
e0117d19-440e-5549-a178-a9cb990833ce
DOI
10.1101/2021.06.11.448164
Open publication

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Tools, strains, and strategies to effectively conduct anaerobic and aerobic transcriptional reporter screens and assays in <i>Staphylococcus aureus</i>DOI 10.1101/2021.06.11.448164
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