Article

Developing a Fluorescence Substrate for HRP-Based Diagnostic Assays with Superiorities Over the Commercial ADHP

2023-04-12

Abstract excerpt

The combination of horseradish peroxidase (HRP) and a fluorescence substrate has been attracting great interests in developing sensitive biochemical analysis and immunoassays. 10-acetyl-3,7-dihydroxyphenoxazine (ADHP or Amplex red) is the most commonly used HRP fluorescence substrate in commercial kits in current market, however, it suffers from some drawbacks, such as non-specific reactivity to carboxylesterase a...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: db1b889c-db2c-5687-ae43-10d4ab76bd54
DOI: 10.2139/ssrn.4414725

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Developing a Fluorescence Substrate for HRP-Based Diagnostic Assays with Superiorities Over the Commercial ADHPDOI 10.2139/ssrn.4414725Read

Evaluating horseradish peroxidase-mimic DNAzyme transducer for glucometer readout: roles of various components and their optimizationSemantic proximity Read

Electrochemiluminescent Detection of hNQO1 and Associated Drug Screening Enabled by Futile Redox Cycle ReactionSemantic proximity Read

Reversible Regulation of Succinate Dehydrogenase by Tools of Photopharmacology.Semantic proximity Read

From Mn-triazine crystalline framework to MnOx@NC: biomimetic double-enzyme activity and labeling of ascorbic acid as well as application in pharmaceuticalsSemantic proximity Read

Principles and applications of green fluorescent protein-based biosensors: a mini-review.Semantic proximity Read

Captamer: A Novel Quantitative Protein Detecting Method Depending on Aptamer-activated Molecular Switches and RPA Signal AmplificationSemantic proximity Read

Dual-Mode Colorimetric and RGB Detection of Sulfide Based on a Magnetic Fe 3 O 4 /CoFe 2 O 4 /Ce-BDC Hybrid NanozymeSemantic proximity Read

Fluorescent Aptasensor Based on Fe3O4@PPySemantic proximity Read

Nitrogen-Doped Biochar Derived from Starch for Enzyme-Free Colorimetric Detection of Uric Acid in Human Body FluidsSemantic proximity Read

Oriented Immobilization of Fab Fragments by Site-Specific Biotinylation at the Conserved Nucleotide Binding Site for Enhanced Antigen DetectionSemantic proximity Read

Chemiluminescent image detection of haptoglobin phenotyping after polyacrylamide gel electrophoresis.Semantic proximity Read

A Biocompatible Dual-AIEgen System without Spectral Overlap for Quantitation of Microbial Viability and Monitoring of Biofilm FormationSemantic proximity Read