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Article

Impact of DNA extraction efficiency on the sensitivity of PCR-based plant disease diagnosis and pathogen quantification

2021-03-01

Abstract excerpt

PCR and qPCR are important methods for plant disease diagnosis and quantification of pathogen populations in host tissues or soil. For most plant diseases, DNA extracted from infected tissue or soil is a prerequisite for PCR or qPCR. The efficiency of DNA extraction should have a direct impact on the sensitivity of PCR and qPCR systems. In this study, plant pathogen DNA extraction efficiencies were evaluated based...

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Literature Corpus work
d791554b-1cf1-5a04-bef6-492fc06a1388
DOI
10.1007/s10658-020-02189-1
Open publication

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Impact of DNA extraction efficiency on the sensitivity of PCR-based plant disease diagnosis and pathogen quantificationDOI 10.1007/s10658-020-02189-1
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