Article
Back to basics: an evaluation of NaOH and alternative rapid DNA extraction protocols for DNA barcoding, genotyping, and disease diagnostics from fungal and oomycete samples.
Molecular ecology resources - 1 Jan 2013
Osmundson Todd W, Eyre Catherine A, Hayden Katherine M, Dhillon Jaskirn, Garbelotto Matteo M
Abstract excerpt
The ubiquity, high diversity and often-cryptic manifestations of fungi and oomycetes frequently necessitate molecular tools for detecting and identifying them in the environment. In applications including DNA barcoding, pathogen detection from plant samples, and genotyping for population genetics and epidemiology, rapid and dependable DNA extraction methods scalable from one to hundreds of samples are desirable....
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