Article

Modular, inducible, and titratable expression systems for <i>Escherichia coli</i> and <i>Acinetobacter baumannii</i>

2024-05-29

Abstract excerpt

Gene expression systems that transcend species barriers are needed for cross-species analysis of gene function. In particular, expression systems that can be utilized in both model and pathogenic bacteria underpin comparative functional approaches that inform conserved and variable features of bacterial physiology. Here, we develop replicative and integrative vectors alongside a novel, IPTG-inducible promoter that...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: d6458cca-b381-54b5-a250-3aa2df102ac4
DOI: 10.1101/2024.05.28.596346

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Modular, inducible, and titratable expression systems for <i>Escherichia coli</i> and <i>Acinetobacter baumannii</i>DOI 10.1101/2024.05.28.596346Read

Gene Expression Platform for Synthetic Biology in the Human Pathogen <i>Streptococcus pneumoniae</i>Semantic proximity Read

Advancing <i>Vibrio</i> genetics: A platform for efficient genomic manipulationSemantic proximity Read

Identification of Promoter Activity in Gene-Less Cassettes from <i>Vibrionaceae</i> SuperintegronsSemantic proximity Read

Functional characterization and optimization of protein expression in <i>Treponema denticola</i> shuttle plasmidsSemantic proximity Read

Molecular basis for the transcriptional regulation of an epoxide-based virulence circuit in <i>Pseudomonas aeruginosa</i>Semantic proximity Read

Development of two compatible plasmids to assess sRNA-mediated post-transcriptional regulation in <i>Acinetobacter baumannii</i>Semantic proximity Read

Physiological Roles of an <i>Acinetobacter</i> -specific σ FactorSemantic proximity Read

Novel genetic modules encoding high-level antibiotic-free protein expression in probiotic lactobacilliSemantic proximity Read

A genetic toolkit for stable transgenesis in the anaerobic gut parasite <i>Blastocystis</i> ST7-BSemantic proximity Read

Equip <i>Fusobacterium nucleatum</i> genetic tool kits with compatible shuttle vectors and engineered intermediatory <i>E. coli</i> strains for enhanced transformation efficiencySemantic proximity Read

Tools, strains, and strategies to effectively conduct anaerobic and aerobic transcriptional reporter screens and assays in <i>Staphylococcus aureus</i>Semantic proximity Read

Genetically diverse uropathogenic <i>Escherichia coli</i> adopt a common transcriptional program in patients with urinary tract infectionsSemantic proximity Read