Article

Long-term single-cell passaging of human iPSC fully supports pluripotency and high-efficient trilineage differentiation capacity

2019-06-06

Abstract excerpt

<h4>ABSTRACT</h4> <h4>Objectives</h4> To validate a straightforward single-cell passaging cultivation method that enables high-quality maintenance of hiPSC without the appearance of karyotypic abnormalities or loss of pluripotency. <h4>Methods</h4> Cells were kept in culture for over 50 passages, following a structured chronogram of passage and monitoring cell growth by population doubling time (PDT) calculatio...

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Literature Corpus work: d551bb45-391f-56c5-ad4f-a1b41cd6fe07
DOI: 10.1101/663047

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