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Long-term single-cell passaging of human iPSC fully supports pluripotency and high-efficient trilineage differentiation capacity

1 Jan 2020

Abstract excerpt

OBJECTIVES: To establish a straightforward single-cell passaging cultivation method that enables high-quality maintenance of human induced pluripotent stem cells without the appearance of karyotypic abnormalities or loss of pluripotency. METHODS: Cells were kept in culture for over 50 passages, following a structured chronogram of passage and monitoring cell growth by population doubling time calculation and cell...

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PMID: 33149912
DOI: 10.1177/2050312120966456

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