Article
Erythropoietin production by the kidney and the liver in response to severe hypoxia evaluated by Western blotting with deglycosylation
2020-03-11
Abstract excerpt
The detection of erythropoietin (Epo) protein by Western blotting has required pre-purification of the sample. We developed a new Western blot method to detect plasma and urinary Epo using deglycosylation. Epo in urine and tissue and erythropoiesis-stimulating agents (ESAs) in urine were directly detected by our Western blotting. Plasma Epo and ESAs were detected by our Western blotting after deglycosylation. The...
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Identifiers and source
- Literature Corpus work
- d4e2a6a0-7570-5004-b348-a00985730eed
- DOI
- 10.1101/2020.03.11.987586