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Optimisation of a multiplexed, high throughput assay to measure neutralising antibodies against SARS-CoV-2 variants

2024-09-04

Abstract excerpt

A multiplexed, lentivirus-based pseudovirus neutralisation assay (pVNT) was developed for high-throughput measurement of neutralising antibodies (nAbs) against three distinct SARS-CoV-2 spike variants. Intra-assay variability was minimised by optimising the plate layout and determining an optimal percentage transduction for the pseudovirus inoculum. Comparison of monoclonal antibody EC 50 titres between single an...

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Literature Corpus work
ceb50363-0fa8-5dd4-be42-913615216d58
DOI
10.1101/2024.09.01.610733
Open publication

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Optimisation of a multiplexed, high throughput assay to measure neutralising antibodies against SARS-CoV-2 variantsDOI 10.1101/2024.09.01.610733
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