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Rapid High Throughput Whole Genome Sequencing of SARS-CoV-2 by using One-step RT-PCR Amplification with Integrated Microfluidic System and Next-Gen Sequencing

2020-11-05

Abstract excerpt

<h4>ABSTRACT</h4> The long-lasting global COVID-19 pandemic demands timely genomic investigation of SARS-CoV-2 viruses. Here we report a simple and efficient workflow for whole genome sequencing utilizing one-step RT-PCR amplification on a microfluidic platform, followed by MiSeq amplicon sequencing. The method uses Fluidigm IFC and instruments to amplify 48 samples with 39 pairs of primers in a single step. Appl...

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Literature Corpus work: c9d68d6b-72e6-5b5c-ab68-ed510ff62ed4
DOI: 10.1101/2020.11.04.369165

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