Article

FRET hybridization probes for the rapid detection of disease resistance alleles in plants: detection of corky root resistance in lettuce

2004-05-01

Abstract excerpt

We describe the development of a non-electrophoresis PCR-based assay for allele discrimination at a disease resistance locus. The assay is based on the emission of light by fluorescence resonance energy transfer (FRET) upon annealing of two hybridization probes. The analysis of melting curve profiles of the probes and templates allowed the detection of single nucleotide polymorphisms. The assay was applied to the...

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Literature Corpus work: c7e9570b-2cd9-575a-b6d3-fb82f6e65108
DOI: 10.1023/b:molb.0000034083.24201.90

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