Article
Microsatellite retrieval in lettuce (Lactuca sativa L.).
Genome - 1 Feb 1999
van de Wiel C, Arens P, Vosman B
Abstract excerpt
By using enriched genomic libraries, microsatellite-containing sequences were isolated from lettuce (Lactuca sativa) with high efficiency. With this approach, a sizeable fraction (up to 55%) of the clones contained a microsatellite. In about half of these clones, primers could be designed for PCR amplification of the microsatellite. This yielded 28 primer sets amplifying unambiguously scorable products, of which...
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