Article

Fluorescence lifetime-based biosensor for monitoring compartmentalized autophagy dynamics in the intact mammalian brain

2025-12-25

Abstract excerpt

Autophagy is a key process in regulation of neuronal development, plasticity, and local metabolism. Yet, autophagy dynamics and regulation within intact neuronal circuits remains poorly understood. Here, we developed a pH sensitive fluorescence lifetime-based imaging method which allows to monitor autophagy dynamics in the living mouse brain. This approach allowed us to uncover compartmentalized autophagic activit...

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Literature Corpus work: c77d7d74-e2fc-579f-b5d0-1f6a7d9cd736
DOI: 10.64898/2025.12.25.693346

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