Article

Broad-Range Virus Detection and Discovery Using Microfluidic PCR Coupled with High-throughput Sequencing

2020-06-12

Abstract excerpt

There is a need for a comprehensive and sensitive method to test for a broad range of viral pathogens in samples without any identifiable pathogen detected. Real-time PCR assays are sensitive and rapid, but their specificity limits their utility in detecting divergent agents. Shotgun high-throughput sequencing methods provide unbiased sequence identification, however, they have limited sensitivity and require comp...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: bf462543-11a6-57d4-a93a-6bb5c80fc6de
DOI: 10.1101/2020.06.10.145052

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Broad-Range Virus Detection and Discovery Using Microfluidic PCR Coupled with High-throughput SequencingDOI 10.1101/2020.06.10.145052Read

ApharSeq: An Extraction-free Early-Pooling Protocol for Massively Multiplexed SARS-CoV-2 DetectionSemantic proximity Read

Viral pathogen discoverySemantic proximity Read

Rapid High-Throughput Whole-Genome Sequencing of SARS-CoV-2 by Using One-Step Reverse Transcription-PCR Amplification with an Integrated Microfluidic System and Next-Generation SequencingSemantic proximity Read

A Universal Next-Generation Sequencing Protocol To Generate Noninfectious Barcoded cDNA Libraries from High-Containment RNA VirusesSemantic proximity Read

High‐throughput microfluidic real‐time PCR as a promising tool in disease ecologySemantic proximity Read

Illumina sequencing of clinical samples for virus detection in a public health laboratorySemantic proximity Read

Rapid cost-effective viral genome sequencing by V-seqSemantic proximity Read

Rapid High Throughput Whole Genome Sequencing of SARS-CoV-2 by using One-step RT-PCR Amplification with Integrated Microfluidic System and Next-Gen SequencingSemantic proximity Read

Virus Identification in Unknown Tropical Febrile Illness Cases Using Deep SequencingSemantic proximity Read

Microfluidic PCR Amplification and MiSeq Amplicon Sequencing Techniques for High-Throughput Detection and Genotyping of Human Pathogenic RNA Viruses in Human Feces, Sewage, and OystersSemantic proximity Read

Microarray-based detection and genotyping of viral pathogens.Semantic proximity Read

Novel NGS Pipeline for Virus Discovery from a Wide Spectrum of Hosts and Sample TypesSemantic proximity Read