Article

An optimized somatic embryo transformation system assisted homozygous edited rubber tree generation method mediated by CRISPR/Cas9

2024-03-16

Abstract excerpt

Previously, we have realized the CRISPR/Cas9-RNP and plasmid mediated protoplast transient transformation genome editing in the rubber tree ( Hevea brasiliensis ), but no gene editing plants were acquired due to the bottleneck of genetic transformation. In present study, antibiotic sensitivity tests against kanamycin, hygromycin and basta were analyzed for embryo screening, the results demonstrated that 10 mg/L h...

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Literature Corpus work: bc4cba17-474a-5285-abf3-ed9370aff830
DOI: 10.1101/2024.03.14.585007

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