Article

Production and characterization of virus-free, CRISPR-CAR T cells capable of inducing solid tumor regression

2021-08-08

Abstract excerpt

<h4>Background</h4> Chimeric antigen receptor (CAR) T cells traditionally harbor viral vector-based sequences that encode the CAR transgene in the genome. These T cell products have yet to show consistent anti-tumor activity in patients with solid tumors. Further, viral vector manufacturing is resource intensive, suffers from batch-to-batch variability, and includes several animal components, adding regulatory an...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: baef399e-31a7-5623-9619-0721b807b982
DOI: 10.1101/2021.08.06.455489

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Production and characterization of virus-free, CRISPR-CAR T cells capable of inducing solid tumor regressionDOI 10.1101/2021.08.06.455489Read

Production and characterization of virus-free, CRISPR-CAR T cells capable of inducing solid tumor regressionSemantic proximity Read

Non-viral expression of chimeric antigen receptors with multiplex gene editing in primary T cellsSemantic proximity Read

Generating universal anti-CD19 CAR T cells with a defined memory phenotype by CRISPR/Cas9 editing and safety evaluation of the transcriptome.Semantic proximity Read

Homology-independent targeted insertion (HITI) enables guided CAR knock-in and efficient clinical scale CAR-T cell manufacturingSemantic proximity Read

Review: Sustainable Clinical Development of CAR-T Cells – Switching From Viral Transduction Towards CRISPR-Cas Gene EditingSemantic proximity Read

Engineering the next-generation of CAR T-cells with CRISPR-Cas9 gene editingSemantic proximity Read

Long Terminal Repeat CRISPR-CAR-Coupled “Universal” T Cells Mediate Potent Anti-leukemic EffectsSemantic proximity Read

Efficient Gene Editing of CART Cells with CRISPR-Cas12a for Enhanced Antitumor EfficacySemantic proximity Read

Fast, efficient and virus-free generation of <i>TRAC</i> -replaced CAR T cellsSemantic proximity Read

Genome-wide CRISPRa screens nominate modulators of CAR T cell survival within distinct tumor cytokine milieusSemantic proximity Read

Efficient nonviral integration of large transgenes into human T cells using Cas9-CLIPTSemantic proximity Read

Explorations of CRISPR/Cas9 for improving the long-term efficacy of universal CAR-T cells in tumor immunotherapySemantic proximity Read