Article

Characterization of deglycosylated anti-cocaine mAb by differential scanning fluorimetry and fluorescence labeling

2025-10-21

Abstract excerpt

A humanized anti-cocaine mAb (h2E2) under development for the treatment of cocaine use disorders was deglycosylated enzymatically, and analyzed by differential scanning fluorimetry (DSF) using two distinct extrinsic dyes, and by FITC fluorescent labeling, to assess domain stability changes induced by deglycosylation of the Fc domain. The deglycosylated Fc fragment of the mAb was generated by proteolysis, and purif...

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Literature Corpus work: afc230ae-be34-5b4f-8e36-e476f9727617
DOI: 10.1101/2025.10.21.683661

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