Article

Construction of a bacterial artificial chromosome library containing large EcoRI and HindIII genomic fragments of lettuce

1997-03-01

Abstract excerpt

Existing bacterial artificial chromosome (BAC) vectors were modified to have unique EcoRI cloning sites. This provided an additional site for generating representative libraries from genomic DNA digested with a variety of enzymes. A BAC library of lettuce was constructed following the partial digestion of genomic DNA with HindIII or EcoRI. Several experimental parameters were investigated and optimized. The BAC li...

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Literature Corpus work: ab2c7fa3-e96e-538e-b4b0-699b54f4094f
DOI: 10.1007/s001220050428

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