Article
A Blumeria graminisf.sp. hordei BAC library--contig building and microsynteny studies.
Current genetics - 1 Nov 2002
Pedersen Carsten, Wu Boqian, Giese Henriette
Abstract excerpt
A bacterial artificial chromosome (BAC) library of Blumeria graminis f.sp. hordei, containing 12,000 clones with an average insert size of 41 kb, was constructed. The library represents about three genome equivalents and BAC-end sequencing showed a high content of repetitive sequences, making contig-building difficult. To identify overlapping clones, several strategies were used: colony hybridisation, PCR...
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