Article

Improved thermostability of creatinase from Alcaligenes Faecalis through non-biased phylogenetic consensus-guided mutagenesis

2020-09-28

Abstract excerpt

<title>Abstract</title> <p><bold>Background: </bold>Enzymatic quantification of creatinine has become an essential method for clinical evaluation of renal function. Although creatinase (CR) is frequently used for this purpose, its poor thermostability severely limits industrial applications. Herein, we report a novel creatinase from <italic>Alcaligenes faecalis </italic>(<italic>af</italic>CR) with higher catalyt...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 9ed08d8b-e1e6-57b0-b856-03ee3d458775
DOI: 10.21203/rs.3.rs-49252/v2

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Improved thermostability of creatinase from Alcaligenes Faecalis through non-biased phylogenetic consensus-guided mutagenesisDOI 10.21203/rs.3.rs-49252/v2Read

A combinatorial multi-site directed mutagenesis solution for improved thermal stability of Lactobacillus plantarum tannase.Semantic proximity Read

Engineered Oxalate decarboxylase boosts activity and stability for biological applicationsSemantic proximity Read

Further characterization and engineering of a 11-amino acid motif for enhancing recombinant protein expressionSemantic proximity Read

Utilization of low-stability variants in protein evolutionary engineering.Semantic proximity Read

Thermal Stabilization of a Bacterial Zn(II)-Dependent Phospholipase C through Consensus Sequence Design.Semantic proximity Read

The consensus concept for thermostability engineering of proteins.Semantic proximity Read

Thermostabilization of Membrane Proteins by Consensus Mutation: A Case Study for a Fungal Δ8-7 Sterol Isomerase.Semantic proximity Read

A thermostable phospholipase C obtained by consensus designSemantic proximity Read

Structural and biophysical studies of new L-asparaginase variants: lessons from random mutagenesis of the prototypic Escherichia coli Ntn-amidohydrolase.Semantic proximity Read

Comprehensive analysis of surface charged residues involved in thermal stability in Alicyclobacillus acidocaldarius esterase 2.Semantic proximity Read

Probing the role of oligomerization in the high thermal stability of Pyrococcus furiosus ornithine carbamoyltransferase by site-specific mutants.Semantic proximity Read

Probing the enzymatic activity and maturation process of the EcAIII Ntn-amidohydrolase using local random mutagenesis.Semantic proximity Read