Article

Refined and benchmarked homemade media for cost-effective, weekend-free human pluripotent stem cell culture

2025-09-19

Abstract excerpt

<h4>Background: </h4> Cost-effective, practical, and reproducible culture of human pluripotent stem cells (hPSCs) is required for basic and translational research. Basal 8 (B8) has emerged as a cost-effective solution for weekend-free and chemically-defined hPSC culture. However, the requirement to home-produce some recombinant growth factors for B8 can hinder access and reproducibility. Moreover, we found the pub...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 97bfa695-00c3-5bcb-95b5-cebfaedf0f63
DOI: 10.12688/openreseurope.18245.2

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Refined and benchmarked homemade media for cost-effective, weekend-free human pluripotent stem cell cultureDOI 10.12688/openreseurope.18245.2Read

Refined home-brew media for cost-effective, weekend-free hiPSC culture and genetic engineeringSemantic proximity Read

Negligible-Cost and Weekend-Free Chemically Defined Human iPSC CultureSemantic proximity Read

Small Molecule-Assisted, Line-Independent Maintenance of Human Pluripotent Stem Cells in Defined ConditionsSemantic proximity Read

Development and evaluation of a novel xeno-free culture medium for human-induced pluripotent stem cellsSemantic proximity Read

Chemically Defined and Xeno-free Culturing Protocol for Human Extended Pluripotent Stem CellsSemantic proximity Read

A robust, reproducible, accessible and scalable protocol for generating three-dimensional human gastruloidsSemantic proximity Read

Production of Human Pluripotent Stem Cell Therapeutics under Defined Xeno-free Conditions: Progress and ChallengesSemantic proximity Read

A Brief History of Cell Culture: From Harrison to Organs-on-a-ChipSemantic proximity Read

Enhancing the Fitness of Embryoid Bodies and Organoids by Chemical CytoprotectionSemantic proximity Read

Robust Expansion of Human Pluripotent Stem Cells: Integration of Bioprocess Design With Transcriptomic and Metabolomic CharacterizationSemantic proximity Read

Recent Trends in Research with Human Pluripotent Stem Cells: Impact of Research and Use of Cell Lines in Experimental Research and Clinical TrialsSemantic proximity Read

Chemically Defined Culture and Cardiomyocyte Differentiation of Human Pluripotent Stem Cells.Semantic proximity Read