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Chemically Defined Culture and Cardiomyocyte Differentiation of Human Pluripotent Stem Cells.

Current protocols in human genetics - 6 Oct 2015

Burridge Paul W, Holmström Alexandra, Wu Joseph C

Abstract excerpt

Since the first discovery that human pluripotent stem cells (hPS cells) can differentiate to cardiomyocytes, efforts have been made to optimize the conditions under which this process occurs. One of the most effective methodologies to optimize this process is reductionist simplification of the medium formula, which eliminates complex animal-derived components to help reveal the precise underlying mechanisms. Here...

Topics

Cell Culture Techniques
Cell Differentiation
Flow Cytometry
Humans
Immunophenotyping
Induced Pluripotent Stem Cells
Myocytes, Cardiac
Phenotype
Pluripotent Stem Cells

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 26439715
DOI: 10.1002/0471142905.hg2103s87
PMCID: PMC4597313

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