Article

A gentle urea-based hybridisation chain reaction (HCR) protocol for use in hymenopteran ovaries

2025-12-05

Abstract excerpt

<title>Abstract</title> <p> <italic>In situ</italic> hybridisation is essential for visualising gene expression, yet many insect tissues are sensitive to standard protocols, especially those using formamide. Here, we present a modified hybridisation chain reaction (HCR) protocol that replaces formamide with urea to improve tissue morphology while maintaining strong and specific signal detection. Using ovaries f...

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Literature Corpus work: 966ab974-7236-5bd6-bdd5-4697f7dd0585
DOI: 10.21203/rs.3.rs-8159582/v1

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A gentle urea-based hybridisation chain reaction (HCR) protocol for use in hymenopteran ovariesDOI 10.21203/rs.3.rs-8159582/v1Read

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