Article

Implementation of an artificial reaction control in a TaqMan method for PCR detection of Ralstonia solanacearum race 3 biovar 2

2009-07-01

Abstract excerpt

A previously published TaqMan PCR test for R. solanacearum race 3 biovar 2 was modified to enable both the validation of negative results and the confirmation of positive results in a closed-tube system. Negative results were validated through the use of a reaction control plasmid, designated pRB2C2, which was designed to generate a 94bp product using the same amplimers targeting the primary diagnostic 68bp sequen...

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Literature Corpus work: 965e883d-e96a-5247-9ecd-8cf119852da3
DOI: 10.1007/s10658-008-9427-6

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