Article

Refined fully automated RT-qPCR assay for simultaneous detection of SARS-CoV-2, Influenza A/B, and RSV with target optimization for improved variant resilience

2025-10-31

Abstract excerpt

<h4>Background</h4> Continuous viral evolution is likely to cause a loss of performance or failure of existing diagnostic assays. Here, we delineate the adaptation and validation of an operational laboratory developed (LDT) multiplex RT-qPCR for simultaneous detection of SARS-CoV-2, influenza A/B (FluA/B) and respiratory syncytial virus (RSV) on a high-throughput, fully automated platform. Furthermore, we explore...

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Literature Corpus work: 8dad01fa-e0c7-5ef1-a634-d6e12a7c790a
DOI: 10.1101/2025.10.29.25339068

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