Article

Quick and easy: 1-day-protocol for detection of normal alternative splicing events and aberrant spliceogenic transcripts with deep RNA sequencing

2021-03-14

Abstract excerpt

RNA sequencing is a promising technique for detecting normal and aberrant RNA isoforms. Here, we present a new single-gene, straightforward 1-day hands-on protocol for detection of splicing alterations with deep RNA sequencing from blood. We have validated our method's accuracy by detecting all normal splicing isoforms of STK11 gene that were previously published. Additionally, the same technique was used to provi...

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Literature Corpus work: 7e58af97-f39a-5d47-a204-22b66d81d230
DOI: 10.22541/au.161574220.03730539/v1

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