Article

Detection of tetracycline-resistance determinants by multiplex polymerase chain reaction in Edwardsiella tarda isolated from fish farms in Korea

2004-10-01

Abstract excerpt

To determine and discriminate the types of tetracycline (Tc)-resistance determinants (tet), we developed a multiplex polymerase chain reaction (PCR). With minimized numbers of primers derived from the variable and conserved regions of six different types of tet genes, tet(A)-(E) and (G), the multiplex PCR was sensitive and specific enough to discriminate the various types of tet genes, even multiple tet genes in a...

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Literature Corpus work: 7d573e5f-d561-53dd-93d3-4063c9fa9efb
DOI: 10.1016/j.aquaculture.2004.07.025

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