Article
Multiplex PCR for the detection of tetracycline resistant genes.
Molecular and cellular probes - 1 Aug 2001
Ng L K, Martin I, Alfa M, Mulvey M
Abstract excerpt
Specific primer pairs were selected for the PCR amplification of 14 tetracycline resistant genes commonly found in Gram positive and Gram negative organisms. Combinations of primer pairs were used in multiplex PCR reactions to detect specific groups of tet genes as follows; Group I tet (B), tet (C), tet (D); Group II tet (A), tet (E), tet (G); Group III tet (K), tet (L), tet (M), tet (O), tet (S); Group IV tetA...
Topics
- DNA Fingerprinting
- DNA Primers
- Electrophoresis, Gel, Pulsed-Field
- Genes, Bacterial
- Genotype
- Humans
- Methicillin Resistance
- Phenotype
- Polymerase Chain Reaction
- Reproducibility of Results
- Salmonella typhimurium
- Staphylococcus aureus
- Tetracycline
- Tetracycline Resistance