Article

Impact of extraction and RT-PCR protocols on stool RNA detection: A study on CRC- associated immune-genes in various patient groups

2024-10-17

Abstract excerpt

<title>Abstract</title> <p>There is a growing interest in using fecal mRNA transcripts as biomarkers for non-invasive detection of colorectal cancer (CRC). The following study compares different RNA extraction and reverse transcription PCR (RT-PCR) methods for mRNA detection in stool and identifies a robust and sensitive protocol. A combination of the Stool total RNA purification kit (Norgen) and the Superscript...

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Literature Corpus work: 7ce15253-2847-5f8d-8219-9b225d701963
DOI: 10.21203/rs.3.rs-4962499/v1

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