Article

Efficient Whole-Genome Sequencing of Monkeypox Virus Using a Novel Nuclease-Multiple Displacement Amplification Enrichment Method

2025-09-10

Abstract excerpt

<title>Abstract</title> <p>Monkeypox virus (MPXV) has a large double-stranded DNA genome (~ 200 kb), which presents challenges for whole-genome sequencing. Conventional enrichment methods have limitations, such as high cost or vulnerability to viral mutations. To address these issues, we developed a novel enrichment strategy combining nuclease treatment and multiple displacement amplification, along with terminal...

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Literature Corpus work: 7328ea90-23a1-5f79-a9e7-d21ed3c5ab75
DOI: 10.21203/rs.3.rs-7498557/v1

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