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Detection of Pantoea stewartii from sweet corn leaves by loop-mediated isothermal amplification (LAMP)

2015-05-01

Abstract excerpt

To improve the diagnosis of Stewart’s wilt, we developed a loop-mediated isothermal amplification (LAMP) assay, based on two conserved sequences of the cpsD and pstS–glmS gene regions. The detection limit of the LAMP assay was 10⁴colony-forming units/mL. No cross reaction was observed with other Pantoea spp., other genera associated with sweet corn diseases, or strains isolated from the surface of maize leaves in...

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Literature Corpus work
71a7b95d-1e5e-5a43-a51e-3ed8cf5a44f2
DOI
10.1007/s10327-015-0580-4
Open publication

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Detection of Pantoea stewartii from sweet corn leaves by loop-mediated isothermal amplification (LAMP)DOI 10.1007/s10327-015-0580-4
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