Article
Development and application of a rapid and visual loop-mediated isothermal amplification for the detection of Sporisorium scitamineum in sugarcane.
Scientific reports - 1 Apr 2016
Su Yachun, Yang Yuting, Peng Qiong, Zhou Dinggang, Chen Yun, Wang Zhuqing, Xu Liping, Que Youxiong
Abstract excerpt
Smut is a fungal disease with widespread prevalence in sugarcane planting areas. Early detection and proper identification of Sporisorium scitamineum are essential in smut management practices. In the present study, four specific primers targeting the core effector Pep1 gene of S. scitamineum were designed. Optimal concentrations of Mg(2+), primer and Bst DNA polymerase, the three important components of the...
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