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Development of a real-time PCR assay for the detection of cow and donkey milk

2012-07-01

Abstract excerpt

A real-time PCR allelic discrimination TaqMan assay based on the analysis of a single nucleotide polymorphism enabling the differentiation of cow (Bos taurus) and donkey (Equus asinus) milk was developed. Specific primers and probes were designed on the mitochondrial cytochrome c oxidase subunit I gene. The primers were designed upstream and downstream the chosen diagnosis site in a conserved region. Two probes we...

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Literature Corpus work
692e6629-4eb5-59ca-97a7-beef3a899593
DOI
10.1007/s00217-012-1732-9
Open publication

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Development of a real-time PCR assay for the detection of cow and donkey milkDOI 10.1007/s00217-012-1732-9
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