Article

An international, interlaboratory ring trial confirms the feasibility of an open-source, extraction-less “direct” RT-qPCR method for reliable detection of SARS-CoV-2 RNA in clinical samples

2021-04-14

Abstract excerpt

Reverse transcription–quantitative polymerase chain reaction (RT-qPCR) is used worldwide to test and trace the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). “Extraction-less” or “direct” real time–reverse transcription polymerase chain reaction (RT-PCR) is an open-access qualitative method for SARS-CoV-2 detection from nasopharyngeal or oral pharyngeal samples with the potential to genera...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 618cf3ce-3026-58ad-9846-ef034f5dfe1c
DOI: 10.1101/2021.04.10.21254091

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

An international, interlaboratory ring trial confirms the feasibility of an open-source, extraction-less “direct” RT-qPCR method for reliable detection of SARS-CoV-2 RNA in clinical samplesDOI 10.1101/2021.04.10.21254091Read

Direct RT-qPCR detection of SARS-CoV-2 RNA from patient nasopharyngeal swabs without an RNA extraction stepSemantic proximity Read

Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extractionSemantic proximity Read

Ultrafast RNA extraction-free SARS-CoV-2 detection by direct RT-PCR using a rapid thermal cycling approachSemantic proximity Read

Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extractionSemantic proximity Read

Open-source RNA extraction and RT-qPCR methods for SARS-CoV-2 detectionSemantic proximity Read

Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCRSemantic proximity Read

A simple RNA preparation method for SARS-CoV-2 detection by RT-qPCRSemantic proximity Read

Rapid direct nucleic acid amplification test without RNA extraction for SARS-CoV-2 using a portable PCR thermocyclerSemantic proximity Read

SARS-CoV-2: Comparative analysis of different RNA extraction methodsSemantic proximity Read

Comparative Analysis of Four Commercial RT-PCR Diagnostic Assay for Detection of Covid-19Semantic proximity Read

An Open One-Step RT-qPCR for SARS-CoV-2 detectionSemantic proximity Read

Comparative effects of viral transport medium heat inactivation upon downstream SARS-CoV-2 detection in patient samplesSemantic proximity Read