Article

Isolation of highly purified genomic material from mitochondria of muscle tissue cells

2022-12-12

Abstract excerpt

In this work, we adapted a method for isolation of a highly purified fraction of mitochondrial DNA from muscle tissues suitable for further sample preparation of libraries without an amplification step for sequencing tasks on various NGS platforms and a method for evaluating the purity of DNA from contamination by nuclear genome regions. We optimized several techniques 7,8 for enrichment of the mitochondrial frac...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 5f159f7d-21b0-5d03-86ab-ca091effbadf
DOI: 10.1101/2022.12.12.520064

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Isolation of highly purified genomic material from mitochondria of muscle tissue cellsDOI 10.1101/2022.12.12.520064Read

PCR-Free Enrichment of Mitochondrial DNA from Human Blood and Cell Lines for High Quality Next-Generation DNA Sequencing.Semantic proximity Read

Accurate and comprehensive analysis of single nucleotide variants and large deletions of the human mitochondrial genome in DNA and single cells.Semantic proximity Read

A simple method for sequencing the whole human mitochondrial genome directly from samples and its application to genetic testing.Semantic proximity Read

Mitochondrial DNA Sequencing and Heteroplasmy Quantification by Next Generation Sequencing.Semantic proximity Read

URMD-Seq: A high-throughput method for scalable detection of ultra-rare mutations in the human mitochondrial genome.Semantic proximity Read

Analyzing Low-Level mtDNA Heteroplasmy-Pitfalls and Challenges from Bench to Benchmarking.Semantic proximity Read

A bioinformatics pipeline for identifying homoplasmic and heteroplasmic mitochondrial DNA SNVs in single-cell RNA-Seq datasets.Semantic proximity Read

Combined MITOchondrial-NUCLEAR (MITO-NUCLEAR) Analysis for Mitochondrial Diseases Diagnosis: Validation and Implementation of a One-Step NGS Method.Semantic proximity Read

Sensitive detection of mitochondrial DNA variants for analysis of mitochondrial DNA-enriched extracts from frozen tumor tissue.Semantic proximity Read

Isolation of Genomic DNA from Mammalian Cells and Fixed TissueSemantic proximity Read

An automated, high-throughput methodology optimized for quantitative cell-free mitochondrial and nuclear DNA isolation from plasmaSemantic proximity Read

Unique molecular identifier-based single strand mitochondrial DNA sequencing for rare substitution mutationsSemantic proximity Read