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Article

A simple method for sequencing the whole human mitochondrial genome directly from samples and its application to genetic testing.

Scientific reports - 22 Nov 2019

Yao Yue, Nishimura Motoi, Murayama Kei, Kuranobu Naomi, Tojo Satomi, Beppu Minako, Ishige Takayuki, Itoga Sakae, Tsuchida Sachio, Mori Masato, Takayanagi Masaki, Yokoyama Masataka, Yamagata Kazuyuki, Kishita Yoshihito, Okazaki Yasushi, Nomura Fumio, Matsushita Kazuyuki, Tanaka Tomoaki

Abstract excerpt

Next-generation sequencing (NGS) is a revolutionary sequencing technology for analyzing genomes. However, preprocessing methods for mitochondrial DNA (mtDNA) sequencing remain complex, and it is required to develop an authenticated preprocessing method. Here, we developed a simple and easy preprocessing method based on isothermal rolling circle mtDNA amplification using commercially available reagents. Isothermal...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 31757988
DOI: 10.1038/s41598-019-53449-y
PMCID: PMC6874554

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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